Team:ECNUAS/Basic Parts
Basic Parts
Favorite basic part:
Biology
Basic part BBa_K3704000 is the coding sequence of glucocorticoid receptor (GR). GR belongs to the steroid hormone receptor subclass of nuclear receptors, which controls physiological processes by activating and inhibiting specific target genes (PMID: 15379669). The ligand-activated receptor dimer activates gene expression by binding to GRE in the promoter region of the glucocorticoid regulatory gene.
Verify the function GR using a luciferase reporting system
In order to test the function of the GR, recombinant GR producing construct (BBa_K3704002) and a luciferase reporting system (two constructs contain parts BBa_K3704001 and BBa_K3522012, respectively) were co-transfected into HEK293T cells. With addition of GR agonist (Dexamethasone, Dex) into the cell culture, significant value of luciferase activity was detected (Figure 1). While, in the presence of Dex and a GR antagonist (Mifepristone, Mife), the luciferase activity reduced to a value similar to the blank control (DMSO).
Figure 1. Relative luciferase activity in the presence of GR agonist (Dexamethasone, Dex) and GR antagonist (Mifepristone, Mife).
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